Figure 5.

Expression and quantification of IL-1β: Quantification of cells stained with an anti-IL-1β antibody showed that unexposed rats treated with E. coli LPS contained more positive cells compared to saline treated unexposed (*, P < 0.026, Figure 5A). ELISA revealed no group differences in the concentrations of IL-1β (Figure 5B, P > 0.05). Immuno-electron microscopy using the same antibody localized IL-1β in pulmonary intravascular monocytes/macrophages (Figure 5C, arrows and inset).