Figure 6.

Expression and quantification of TNF-α: Quantification of cells stained with anti-TNF-α antibody showed that following E. coli LPS challenge, both unexposed and exposed groups contain more number of positive cells compared to saline treated both exposed and unexposed groups (*, P < 0.05, Figure 6A). Within LPS challenged groups, barn exposed rats contained more cells positive for TNF-α compared to unexposed rats (*, P = 0.005). ELISA on lung homogenates showed no differences in the concentrations of TNF-α among the groups (P > 0.05, Figure 6B).